class: title-slide, center # Tagging cisco: Lessons from the Les Cheneaux Islands ## Todd Hayden, Chris Holbrook .center[<img src="data:image/png;base64,#../images/cisco_drawing.png" width="45%" height="45%"/>] <div class="flex2"> <img src="data:image/png;base64,#../images/MSU.png" width="20%" height="20%"/> <img src="data:image/png;base64,#../images/USGS.png" width="20%" height="20%"/> </div> --- class: top, center # Acknowledgments .left[ - Hammond Bay Biological Station - Michigan Department of Natural Resources - US Fish and Wildlife Service - Real-Time Aquatic Ecosystem Observation Network - Great Lakes Fishery Commission - Great Lakes Acoustic Telemetry Observation System ] .center[ <img src="data:image/png;base64,#../images/icy_night.JPG" width="30%" height="30%" /> ] --- class: top, center # History .left[ - 2017 field tagging trials (tagged few fish, Government Bay) - 2018 preliminary project (28 receivers, 25 V9 tags) - 2019-2021 full project (200 tags, 120 receivers) - 2020 winter- tagging under ice (gill nets) ] <div class="flex2"> <img src="data:image/png;base64,#../images/full_array.png" width="40%" height="40%" /> <img src="data:image/png;base64,#../images/ice_tagging_winter.JPG" width="40%" height="40%" /> </div> --- class: top, center # Capture .left[ - Nighttime electrofishing - Peak spawn- November 10-15 - ~ 84% survival (6 months) - Tagged during peak spawn ~ 10-15' water - Fish not available before/after spawning (too deep!) ] .center[ <img src="data:image/png;base64,#../images/cisco_efish_boat_LesCheneaux_cholbrook_20181108.JPG" width="38%" height="38%"/> ] --- class: top, center # Capture .left[ - Gill net - Difficult or no recovery - Tried under ice gill nets, 20 minute soak, ~ 8% survival (6 months) - Lots of "rope burns" ] .center[ <img src="data:image/png;base64,#../images/inside_ice_shanty_cisco_tagging_winter.JPG" width="18%" height="18%"/> ] --- class: top, center # Post-capture care .left[ - Goal: minimize handling time - Capture 1-10 fish, transfer fish to waiting "transport boat" and Moved to shore - Hold fish in flow-through .red[ROUND] tank (4') with .red[FLOW] - Surgery immediately after fish "recovered" from electrofishing ] .center[ <img src="data:image/png;base64,#../images/cisco_cauldron_bf_tagging.JPG" width="40%" height="40%"/> ] --- class: top, center # Surgery .left[ - Tent, propane heater to prevent ice formation on fish and us! - V9 tags, fish ~ 400 mm TL - TENS electro mobilization - <2 min surgery, 2 interrupted sutures - Constant water flow over gills ] <div class="flex2"> <img src="data:image/png;base64,#../images/tent1.JPG" width="30%" height="30%"/> <img src="data:image/png;base64,#../images/tent2.JPG" width="30%" height="30%"/> <img src="data:image/png;base64,#../images/fish_on_surgery_table.JPG" width="30%" height="30%"/> </div> --- class: top, center # Recovery .left[ - fish released as soon as swimming on own and able to maintain orientation - dedicated nurse- gently "assist" fish when upside down - upside down cisco needs immediate care to prevent death! - cisco are finicky- some fish appeared dead in recovery but swam off after release. ] <div class="flex2"> <img src="data:image/png;base64,#../images/cisco_nurse_assist.JPG" width="30%" height="30%"/> <img src="data:image/png;base64,#../images/recovery_tank.JPG" width="30%" height="30%"/> </div> --- class: top, center # Tanks and flow .left[ - Round tanks with sufficient flow flow is critical - Rectangular/oval/cattle troughs result in "stuck fish" - How much flow? - Fish should be swimming against current away from side ("endless pool") - Not enough water flow- fish turn over upside-down, die - Too much water flow- fish scrape tank sides - We used submersable utility pumps, short hoses and clamps to regulate ] <video src="../images/tagged_cisco_recovery_sm.webm" type="video/webm"> </video> --- class: top, center # Take-home ideas .left[ - Minimize handling, time in tanks - Round tanks are critical - Water flow - TENs for electro immobilization- quick recovery - release immediately after fish has regained ability to swim ] .center[<img src="data:image/png;base64,#../images/cisco_on_surgery_table.JPG" width="40%" height="40%"/>]